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. 2014 Jul 9;23(23):6366–6374. doi: 10.1093/hmg/ddu358

Figure 1.

Figure 1.

Mecp2 is required in OSNs for the refinement of olfactory sensory axon convergence. M72-IRES-taulacZ axons converge in wild-type adult OB, one on the lateral side (A) and one on the medial side (D). In adult Mecp2−/+ heterozygous (B, E) and Mecp2−/y hemizygous OB (C, F). (G). Lack of complete M72 olfactory sensory axon convergence persisted into adulthood in (P56) Mecp2 mutants (G). Serial sections of wild-type OB show M72-IRES-taulacZ axons (green) travel in the olfactory nerve layer and terminate into glomeruli delineated by OMP IF (red) (H, H′). Abnormal fasciculation (arrows) and mistargeting of M72 axons (arrowhead) are observed in Mecp2−/y OSN axons (I–I′′′). Mecp2 expression is ablated in mature OSNs via OMP-Cre-mediated events. In control mice, MeCP2 expression (red in J and J′) is present in OMP expressing (green in J) OSNs. In OMP-Cre-mediated conditional knockout OE, Mecp2 expression (red in K and K′) is not detectable in mature OSNs but is at the same level in sustentacular cells compared with control (arrows in J′ and K′). Supernumerary M72 glomeruli were detected in P56 Mecp2flox/y;OMP-Cre OB (M) but not in Mecp2+/y;OMP-Cre OB (L). Bar = 500 µm in (A)–(F), 50 µm in (H)–(I), 25 µm in (J)–(K) and 250 µm in (L)–(M). Student's t-test *P < 0.05, **P < 0.01.