Figure 4.

(A) CD spectra of pure dissolved bovine serum albumin (thick black line) and in the presence of different concentrations of PVP-coated silver nanoparticles. The concentration of the nanoparticles ranged from 1.20·10¹¹ to 1.91·10¹¹ nanoparticles mL⁻¹. (B) Linear fit to the evaluated data for the determination of a K_D value for these nanoparticles according to the equation given in [63,69]. Note that the accuracy of the computed K_D value is determined by the accuracy of the CD spectra and the resulting values for the occupation of surface sites by the protein.