Figure 6.

Agglomeration of internalized silver nanoparticles in hMSC analyzed by phase contrast microscopy (B), FIB/SEM (C), and EDX (D). hMSC were incubated for 24 h in the presence of 2 µg mL⁻¹ silver acetate (A) as control or 20 µg mL⁻¹ silver nanoparticles (B–D). Accumulated nanoparticles were detected in perinuclear areas (B, white arrow). Cell nuclei were stained with blue-fluorescent Hoechst33342 (A, black arrow; B). A single cell was cross-cut by FIB milling and the cut interface was analyzed by SEM to visualize the internalized particles (C, white arrow). The corresponding EDX spectrum of the sectioned cell shows silver signals (D, grey arrow).