Figure 9.

Localization of silver nanoparticles agglomerates in hMSC. A representative light micrograph after digital contrast enhancement (DCE filter; the black arrow denotes silver accumulation) (A), a fluorescence micrograph (B) and a combination of both (C) are shown. The white arrow denotes the intracellular accumulation of silver particles inside the endo-lysosomes (C). The blue fluorescence of Hoechst33342, the green fluorescence of BODIPY FL C5-ceramide and the red fluorescence of Lyso Tracker Red DND 99, were used as probes of cell nucleus, Golgi complex and endo-lysosomes, respectively.