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letter
. 2014 Nov;99(11):e214–e217. doi: 10.3324/haematol.2014.108555

Figure 3.

Figure 3.

Involvement of platelets in SCA neutrophil-RBC interactions. Percentages of neutrophils from steady-state SCA (on/off HU) individuals aggregated to (A) RBCs (CD235a+), (B) reticulocytes (CD235a+/CD71+) and (C) mature RBCs (CD235a+/CD71−) under basal conditions and after incubation of granulocyte suspension with a non-specific IgG antibody or P-selectin function-blocking antibody (Anti-CD62P; clone 9E1, 2 μg/mL) (n=7); *P<0.05, **P<0.01, compared to basal (Friedman/Dunn’s). Percentages of neutrophils aggregated to (D) RBCs (CD235a+), (E) reticulocytes (CD235a+/CD71+) and (F) mature RBCs (CD235a+/CD71−), and demonstrating the presence (+) or not (−) of activated (P-selectin-positive) platelets in these aggregates when analyzed by flow cytometry (SCA on/off HU; n=7). (G) Representative images of the involvement of platelets (CD62P+; red) in the aggregates of neutrophils (CD66b+; purple) with RBC (CD235a+; yellow) expressing, or not, the transferrin receptor (CD71+; green). BF: brightfield mode and respective channels of fluorescence.