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. 2013 Nov 21;13:258. doi: 10.1186/1471-2148-13-258

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Copyright © 2013 Deakin et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Examples of FISH determining the orientation of adjacent BAC clones on tammar wallaby metaphase chromosomes. Orientation of (A)SERPINA1 labelled green and NUDC2 in red on chromosome 1; (B)RUNX2 in red and MRPS10 in green on chromosome 2 and (C)CORTBP2 in green and p100 in red on chromosome 3. Chromosomes have been counterstained with DAPI. Scale bar represents 10 μm.