Table 1. Hydrogen Deuterium Exchange Reaction Time Tablea.
HDX
reaction time under various pH conditions |
||||||
---|---|---|---|---|---|---|
exchange time corrected to standard condition (pH 7.5, 4 °C) | pH 7.5 (Standard) | pH 7.0 (× 100.5) | pH 6.5 (× 101) | pH 6.0 (× 101.5) | pH 5.5 (× 102) | pH 5.0 (× 102.5) |
3 s | -- | 10 s | 32 s | 100 s | 5 min 16 s | 16 min 40 s |
10 s | 10 s | 32 s | 100 s | 5 min 16 s | 16 min 40 s | 52 min 42 s |
30 s | 30 s | 1 min 35 s | 5 min | 15 min 49 s | 50 min | 2 h 38 min |
1 min | 1 min | 3 min 10 s | 10 min | 31 min 37 s | 1 h 40 min | 5 h 16 min |
2 min | 2 min | 6 min 19 s | 20 min | 1 h 3 min | 3 h 20 min | 10 h 32 min |
6 min | 6 min | 18 min 58 s | 1 h | 3 h 9 min | 10 h | 1 d 7 h |
15 min | 15 min | 47 min 26 s | 2 h 30 min | 7 h 54 min | 1 d 1 h | 3 d 7 h |
1 h | 1 h | 3 h 9 min | 10 h | 31 h 37 min | 4 d 4 h | -- |
The exchange times are corrected to standard condition at pH 7.5 and 4 °C shown in the first column of the table. The numbers in the brackets in columns 2 to 7 are the time conversion factors for corresponding pH conditions.