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. 2014 Oct 6;53(43):6776–6785. doi: 10.1021/bi500861x

Figure 2.

Figure 2

SDS–PAGE gel of the NEMO constructs as labeled: NEMOw, lanes 2–4; NEMOwNcc, lanes 5–7; NEMOwCcc, lanes 8–10; NEMOwDcc, lanes 11–13. MW markers in lane 1 are labeled. The heating temperature used in the preparation of the sample for electrophoresis is indicated as well as the presence or absence of the reducing agent DTT. For each construct, the lower band represents the monomer (MW = 8.8, 11.4, 12.1, and 14.8 kDa) and the upper band the dimer (NEMO constructs run lower than the calculated MW respect to MW standards, under these conditions).