Table 1.
Number of selected lines and plants, PCR positive plants and transformation efficiency of cultivar Wanad transformed with (Pina), (Pinb) silencing cassettes and control pMC161 (pMCG) vector
|
Silencing cassette |
Number of explants |
Number of selected |
PCR positive plants / transformation efficiency [%]
a
|
|
|---|---|---|---|---|
| Lines (SD) | Plants (SD) | |||
| (Pina) |
582 |
17 ± 0.70 |
39 ± 4.94 |
14 / 2.4 |
| (Pinb) |
1460 |
3 ± 1.22 |
17 ± 6.94 |
2 / 0.14 |
| (Pina)+(Pinb) |
1960 |
0 |
0 |
0 / 0.00 |
| (pMCG) |
452 |
8 ± 1.92 |
14 ± 3.41 |
4 / 0.88 |
| Sum /mean | 4454 | 28 ± 1.28 | 70 ± 5.1 | 20 / 1.14 |
aPercentage of initial explants giving rise to PCR positive plants.