Table 2.
Plasmids and strains
|
Plasmids/Strains |
Description |
Source or reference |
|---|---|---|
| Plasmids | ||
| pUC57-glda_sth |
Chemically synthesized gene cassette consisting of Ptrc, gldA101-syn and sth. |
Genewiz;
[20,30,37] |
| pQZ115 |
Plasmid carrying gldA101 |
[20] |
| pTKA3 |
Backbone plasmid for all vectors constructed in this study, with psbA1 as the integration loci. |
[32] |
| pYY1 |
Derived from pTKA3 with gldA101 and the promoter, Ptrc. |
This study |
| pDY2 |
Derived from pTKA3 with gldA101-syn, sth and the promoter, Ptrc. |
This study |
| pDY3 |
Derived from pTKA3 with gldA101-syn and the promoter, Ptrc. |
This study |
|
Strains | ||
|
E. coli 10-Beta |
Cloning host strain. |
New England Biolabs |
|
Synechocystis sp. PCC 6803 |
Glucose tolerant wild type, naturally competent. |
This study |
| AV08 |
Synechocystis Ptrc::gldA101::Kmr, GlyDH* of Bacillus. |
This study |
| AV10 |
Synechocystis Ptrc::(gldA101-syn)-sth::Kmr, GlyDH* of Bacillus, transhydrogenase of Pseudomonas. |
This study |
| AV11 | Synechocystis Ptrc::gldA101-syn::Kmr, GlyDH* of Bacillus. | This study |