Figure 3.

Collection of analytes by integration of microneedle patch into the multiwell microplate. (a) Microneedle patches with a hard backing are affixed to form the bottom of the wells of a bottomless multiwell microplate (1). A drop of water is dispensed to the microneedles (2, 3) to extract/dissolve analytes from the microneedles (4) to enable subsequent in situ analysis. (b) Microneedle patches adhered to the wells of a 96-well microplate. (c) View of dye solution (sulforhodamine) added to two wells and water added to neighboring wells, all sealed on the bottom with microneedle patches, demonstrating lack of material transfer between the wells after incubation for 12 h.