Figure 4.

Restoration of WNT4 in BJAB inhibits cell growth. A) Percentage of cell viability was measured in BJAB-Tet-WNT4 cells after 24- and 48-h incubations with Doxycycline (Doxy). We also included parental BJAB and BJAB-Tet cells as controls. Percentage of cell viability was measured by adding WST-1 to the cell cultures for 2 h and reading the absorbance of treated and untreated cells at 440 nm. The value of each cell line of non-treated cells was set as 100% of cell proliferation. B) BJAB-Tet-WNT4 cells cultivated for 72 h in the presence or absence of Doxy were counted every 24 h. The parental BJAB cell line was included as control.