Table 1.
Oligonucleotides used for the qRT-PCR analysis
| Gene name | Sequence accession number | Primer sequence | Primer location (Exon) | Prod. length | T°a | |
|---|---|---|---|---|---|---|
|
WNT4 ORF
|
Wingless-type MMTV integration site family, member 4 |
NM_030761 |
F GGCACCATGAGTCCCCGCTCG |
99–119 (1) |
1055 |
60 |
|
R GCAGGGCTAGGCAGGCGGTCA |
1158–1178 (5) |
|||||
|
WNT4
|
Wingless-type MMTV integration site family, member 4 |
NM_030761 |
F GGAACTGCTCCACACTCGACTC |
364–385 (3) |
259 |
60 |
|
R CGCACATCCACAAACGACTGT |
602–622 (4) |
|||||
|
MYC
|
V-myc myelocytomatosis viral oncogene homolog (avian) |
NM_002467.4 |
F CCAGCGCCTTCTCTCCGTC |
1208–1226 (2) |
302 |
60 |
|
R GGGAGGCGCTGCGTAGTTGT |
1490–1509 (3) |
|||||
|
JUN
|
Jun proto-oncogene |
NM_002228.3 |
F TGGAAAGTACTCCCCTAACCT |
2786–2806 (1) |
250 |
60 |
|
R CTGAAACATCGCACTATCCTT |
3015–3035 (1) |
|||||
|
FOSL1/FRA1
|
FOS-like antigen 1 |
NM_005438.3 |
F AGGAACCGGAGGAAGGAACTG |
554–574 (3) |
199 |
60 |
|
R TGCCACTGGTACTGCCTGTGT |
732–752 (4) |
|||||
|
AXIN2
|
Axin 2 |
NM_004655.3 |
F AAAAAGGGAAATTATAGGTATTAC |
2678–2701 (10/11) |
277 |
54 |
|
R CGATTCTTCCTTAGACTTTG |
2935–2954 (11) |
|||||
|
CCND1
|
cyclin D1 |
NM_053056.2 |
F CCCCAACAACTTCCTGTCCTAC |
866–887 (4) |
236 |
60 |
|
R GCCCTCAGATGTCCACGTC |
1083–1101 (5) |
|||||
|
SURVIVIN/BIRC5
|
Homo sapiens baculoviral IAP repeat containing 5 |
NM_001012271.1 |
F TGAGCTGCAGGTTCCTTATCTG |
1057–1078 (5) |
234 |
60 |
|
R GAATGGCTTTGTGCTTAGTTTT |
1269–1290 (5) |
|||||
|
RPL32
|
Ribosomal protein L32 |
NM_000994.3 |
F GACTTGACAACAGGGTTCGTAG |
213–234 (3) |
320 |
60 |
|
R ATTTAAACAGAAAACGTGCACA |
511–532 (4) |
|||||
|
RPS18
|
Ribosomal protein S18 |
NM_022551.2 |
F CGATGGGCGGCGGAAAA |
105–121 (2) |
283 |
58 |
|
R CAGTCGCTCCAGGTCTTCACGG |
366–387 (5) |
|||||
|
ACTB
|
Beta Actin |
NM_001101.3 |
F TCCGCAAAGACCTGTACG |
950–967 (5) |
298 |
60 |
| R AAGAAAGGGTGTAACGCAACTA | 1226–1247 (6) |