Table 1. Assignable RNAseq read counts from F1 hybrids and parents.
F1 Hybrid Count | Parent Count | F1 Hybrid Percent of Total | Parent Percent of Total | ||
Total Reads | Ear | 996,210,711 | 286,233,926 | - | - |
Leaf | 1,133,517,167 | 282,553,096 | - | - | |
Stem | 1,211,779,746 | 276,295,164 | - | - | |
Aligned Reads | Ear | 556,387,109 | 171,185,368 | 55.85% | 59.81% |
Leaf | 671,815,900 | 169,564,817 | 59.27% | 60.01% | |
Stem | 716,223,906 | 162,866,225 | 59.11% | 58.95% | |
Segregating Site Reads | Ear | 74,556,872 | 85,296,872a | 7.48% | 29.80%a |
Leaf | 73,094,422 | 78,878,805a | 6.45% | 27.92%a | |
Stem | 91,355,219 | 78,583,423a | 7.54% | 28.44%a |
A higher number and percentage of reads map to segregating sites in parents due to each set of parent reads being used in multiple comparisons. In contrast each of the F1 comparisons can only map to segregating sites between two pseudo-transcriptomes.