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. 2014 Sep 9;3(9):e192. doi: 10.1038/mtna.2014.43

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Copyright © 2014 American Society of Gene & Cell Therapy

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Myelin basic protein (MBP)-binding activity of MBPcl3 aptamer using an ELISA-like assay. 0.5 µg of pure MBP was incubated in a microtiter plate with equal nmol of an unselected aptamer library (Lib35), MBPcl3 or MBPcl9 (a) or with a scrambled MBPcl3 aptamer whose entire nucleotide sequence was randomly rearranged (b). Bound aptamers were detected with horseradish peroxidase (HRP)-conjugated avidin and developed with 3,3',5,5'-tetramethylbenzidine (TMB) substrate (dark gray). Wells without MBP were used as controls (light gray). Experiments were performed in triplicate. The asterisk indicates a significant difference in comparison with MBPcl3 (P < 0.05, Student's t-test). Data are shown as mean ± SD.