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. 2014 Sep 9;3(9):e192. doi: 10.1038/mtna.2014.43

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Copyright © 2014 American Society of Gene & Cell Therapy

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Detection of a myelin basic protein (MBP) peptide with MBPcl3 aptamer. Two micrograms of pure MBP peptide were incubated in a microtiter plate with equal nmol of an unselected aptamer library (Lib35) or MBPcl3. Bound aptamers were detected with HRP-conjugated avidin and developed with 3,3',5,5'-tetramethylbenzidine (TMB) substrate (dark gray). Wells without MBP peptide were used as controls (light gray). Experiments were performed in triplicate. One asterisk indicates a significant difference in comparison with MBPcl3 and two asterisks indicates a significant difference in comparison with Lib35 (P < 0.05, Student's t-test). Data are shown as mean ± SD.