Figure 2.

CCR5-ZFN-expressing NILV transduced activated and resting CD4⁺ T cells resist HIV infection in vitro. (a) activated or (b) resting CD4⁺ T cells were challenged with HIV_BAL 48 hours after transduction and the supernatants collected at different time points were tested by LRT qRT-PCR to determine the viral titers. PBMC and empty NILV were used as controls. All the samples for qRT-PCR were in triplicates and plotted as average copy numbers. One way analysis of variance was used to compare the differences between groups at different time points. Significance levels are shown in the figures as *P < 0.05; **P < 0.01; and ***P < 0.001.