Figure 4.

Direct ZFN transduction of whole PBMCs from treated or untreated seropositive individuals confers protection from endogenous virus in Hu-PBL mice. Whole PBMCs (3 x 10⁶) from indicated subjects were mock-transduced or transduced with HIV env pseudotyped NILV and injected iv into NSG mice. CD4 T-cell counts over time (a) viral loads measured by (b) p24 ELISA, and (c) qRT-PCR in sera collected on day 49 in Hu-PBL mice infused with PBMCs from an ART negative HIV-1⁺ individual. Multiple T test was used to compare the differences between Vector or ZFN treated groups (three mice each group), significance values are shown in the figures. *P < 0.05, **P < 0.01. (d–g) CD4 T-cell counts over time (d,e) and viral load measured by qPCR in sera collected on day 35 (f,g) in Hu-PBL mice infused with CD4 T cells from two ART treated HIV positive individuals. Multiple t-test was used to compare the differences between Vector (n = 4 for S24 and n = 5 for S30) and ZFN (N = 7 for both S24 and S30) treated groups. *P < 0.05, **P < 0.01.