Fig. 1.

Uptake of fluorescently labeled NPs into EAhy926 cells. Images showing samples used for the quantification of uptake by fluorometry. Cells show uptake of red fluorescently labeled CPS20 particles suspended in DMEM without FBS and in DMEM + 10% FBS (a) and of red fluorescently labeled MCNT8 and MCNT50c suspended in DMEM + 10% FBS (b). Scale bar: 20 μm. For co-localization studies, green fluorescently labeled NPs were used to allow discrimination of particles and mitochondria identified by the red fluorescent dye MitoTracker CMXRos. Localization of CPS20 (c) and MCNT8 particles (d) is shown. Nuclei were counterstained with Hoechst33342 (blue).