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. Author manuscript; available in PMC: 2014 Nov 6.
Published in final edited form as: Soft Matter. 2012;8(42):10877–10886. doi: 10.1039/C2SM25702E

Fig. 2.

Fig. 2

Another example of a fluorescently labeled GUV attached to a glass slide covered by a hydrophobic SAM. A selection of images from Movie M2 in the ESI† show fluorescence images focused at the glass– water interface. (A) A sequence of fluorescence images. Slides (a) and beyond: a GUV immobilized at the surface. Slides (b)–(f): a hemifusion pore formed near the membrane contact site. Slides (d)–(f): a small vesicle trapped inside the GUV was expelled from the GUV through the pore. (B) Angularly averaged intensity profile (dotted line) and its radial derivative (solid line) of the image shown in slide (i) of panel A. The boundaries of the distinct hemifusion zones are indicated by peaks in the derivative function and marked with a dashed vertical line.