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. Author manuscript; available in PMC: 2014 Nov 6.
Published in final edited form as: J Neuroimmunol. 2010 Sep 14;226(0):8–19. doi: 10.1016/j.jneuroim.2010.05.026

Figure 6.

Figure 6

Androgen replacement in orchidectomized male β2m−/− mice decreases LCMV-specific T cell responses in the cervical LN. Groups of male β2m−/− mice were orchidectomized and either sham-operated (Orch; white bars, n = 11 except figure E: n = 7) or implanted with DHT pellets (Orch DHT 0.5 mg and Orch DHT 5 mg; black bars, n = 5 for each dose except figure E: n = 6 for each dose). Animals were infected i.c. with 1 × 103 pfu LCMV, sacrificed at day 8 of infection, and perfused with 10 mL PBS. Mononuclear cells from cervical LN and brains were isolated, stained with I-Ab gp61-80 tetramer, and analyzed by flow cytometry. Percentages (A, C) and total numbers (B, D) of tetramer-positive cells in the cervical LN (A, B) and brain (C, D) of infected sham-operated orchidectomized males and infected DHT-treated orchidectomized males. IFN-γ levels in the serum (E) and CSF (F). Data represent the mean ± SEM and are pooled from two independent experiments. *p ≤ 0.02 (Mann-Whitney test, Minitab for Windows).