DHT treatment of female β2m−/− mice decreases LCMV-specific T cell responses in the cervical LN. Groups of female β2m−/− mice were sham-operated (Sham F; white bars, n = 6) or implanted with DHT pellets (DHT; black bars, n = 6), infected i.c. with 1 × 103 pfu LCMV, sacrificed at day 8 of infection, and perfused with 10 ml PBS. Mononuclear cells from cervical LN and brains were isolated, stained with I-Ab gp61-80 tetramer, and analyzed by flow cytometry. Percentages (A, C) and total numbers (B, D) of tetramer-positive cells in the cervical LN (A, B) and brain (C, D) of infected sham-operated females and infected DHT-treated females. INF-γ levels in the serum (E) and CSF (F). Data represent the mean ± SEM and are representative of two independent experiments. *p ≤ 0.03 (Mann-Whitney test, Minitab for Windows).