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. 2013 Nov 21;12:172. doi: 10.1186/1475-2840-12-172

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Copyright © 2013 Ramírez et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Anti-oxidant effects of eplerenone in ZDF hearts and HF-stimulated cardiomyocytes. (A) DHE and Mitosox staining (red) in ZDF and ZDF-treated myocardia. Nuclear labelling by DAPI (blue) is also shown. N = 6, each group. (B) By flow cytometry (left), mitosox staining in 0-60 min HF and HF+eplerenone (10^-6 M) incubated cardiomyocytes. By immunofluorescence (right), mitosox staining after 10 min of HF stimulation (0.12 mM). (C) ATP production in 12 h HF-stimulated cardiomyocytes. *p < 0.05 and **p < 0.01 vs. control. †p < 0.05 and ††p < 0.01 vs. related ZDF or HF.