Table 5. Genome differences specific for the TEN Bosnia A strain^a.

	Non-coding sequences (altogether 44 bp)		Coding sequences (altogether 2728 bp)
Nucleotide difference	Number of differences	Number of affected nucleotides	Number of differences	Number of affected nucleotides	Affected gene	Predicted proteinb	Function
deletion	1	1×13 bp	9	1× single bp	TENDBA_0126bc	HP	Unknown
		(altogether 13 bp)		1×2 bp	TENDBA_1029c	TCHP	Unknown
				1×2300 bp	TENDBA_0316c	TprGI	Virulence
				(altogether 2303 bp of protein truncation or elongation)
				2×3 bp	TENDBA_0136d	TCHP	Virulence
					TENDBA_0225	CHP	Unknown
				1×4 bp	TENDBA_0548	TCHP	Unknown
				1×6 bp	TENDBA_0859	TCHP	Unknown
				1×9 bp	TENDBA_0067	CHP	Unknown
				1×24 bp	TENDBA_0865	TCHP	Unknown
				(altogether 49 bp of protein shortening)
insertion	0		5	1× single bp	TENDBA_0082ac	HP	Unknown
				(altogether 1 bp of protein truncation due to frameshift mutation)
				3×3 bp	TENDBA_0859 (2x)	TCHP	Unknown
					TENDBA_0898	RecB	DNA replication, repair and recombination
				1×6 bp	TENDBA_0856	TCHP	Unknown
				(altogether 15 bp of protein elongation)
substitution	31		356	197 different aa	TENDBA_0136d	TCHP	Virulence
			(TPA identical with TPE)	122 identical aa	TENDBA_0548	TCHP	Unknown
			4		TENDBA_0856	TCHP	Unknown
			(TPA different from TPE)		TENDBA_0859	TCHP	Unknown
					TENDBA_0865	TCHP	Unknown

^aThe tprK gene (TENDBA_0897) was excluded from this list of differences because of high intra-strain sequence diversity.

^bHP – hypothetical protein, CHP – conserved hypothetical protein, TCHP – treponemal conserved hypothetical protein.

^csee also Table 2.

^dTPA and TPE orthologs to TENDBA_0136 have been experimentally shown to bind human fibronectin [28].