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. 2014 Nov 6;8(11):e3183. doi: 10.1371/journal.pntd.0003183

Figure 1. Suppression of c-Jun leads to down-regulation of AP-1 activity.

Figure 1

A, Flag-tagged dominant-negative mutant of c-Jun, lacking the first 169 amino acid transactivation domain (Flag-Δ169) was introduced into T. annulata-infected macrophages. B, Suppression of c-Jun reduces AP-1 activity in T. annulata-infected macrophages, as measured by 3XTRE-driven luciferase activity (TRE-luc). The activity of AP-1 is 20 times lower in the vaccine line (V-Delta 169) compared to the control line (V). C, Western blot analysis of the expression of two different AP-1 family members in vaccine and control lines showing that expression of c-Jun is decreased and ATF2 increased in vaccine line. In spite of an increase in ATF2 protein the level of phospho-ATF2 (p-ATF2) is markedly reduced. Protein amounts were compared to actin and decreases in p-ATF2 and c-Jun quantified using Image J (C1 and C2).