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. 2014 Nov 6;8(11):e3183. doi: 10.1371/journal.pntd.0003183

Figure 3. Inhibition of c-Jun results in reduced metastatic potential in vitro.

Figure 3

A, RT-qPCR determination of relative expression shows adam19 and mmp9 are diminished and timp3 levels are augmented in V-Delta 169 macrophages. B, MMP9 and ADAM19 protein levels are also decreased in V-Delta 169 macrophages. Amounts of actin were used as loading control. C, Gelatin zymography demonstrates that MMP9 proteinase activity is reduced in supernatants of V-Delta 169 macrophages. D, The invasive capacity, as determined by Boyden chamber assays show that V-Delta 169 macrophages have a 55% reduction in their ability to traverse Matrigel compared to virulent Jed 4 macrophages.