Figure 2. Effect of ouabain and hypoxia on intracellular Na⁺, K⁺ and ATP concentrations.

RASMC were incubated for 24 hr under normal oxygen partial pressure (5% CO₂/air - control) ±3 µM ouabain or exposure to hypoxia (5% CO₂/95% N₂)/glucose deprivation in normal high-Na⁺, low-K⁺ ([Na⁺]_o/[K⁺]_o = 140/5) or in low-Na⁺, high-K⁺ DMEM-like medium ([Na⁺]_o/[K⁺]_o = 131/115). Means ± S.E. from 3 independent experiments performed in quadruplicate are shown. *p<0.05 compared to the controls.