Figure 5. Rate constants of the axial ligands replacement and corrin ring destruction of dicyanocobinamide as a function of HOCl concentration.

Upper panel, the observed rate constants of (OCl)(CN)-Cbi complex formation (open circles) and its conversion to (OCl)₂-Cbi (monitored at 613 nm) (closed circles) observed in Fig. 3 were plotted as a function of HOCl concentration. R² values for the first and second phases were 0.992 and 0.995 respectively. Data represent the mean of triplicate determinations from an experiment performed three times. Lower panel, the rate constants for the corrin ring destruction, for the same reaction, monitored at 493 nm as a function of HOCl concentration. R² value was found to be 0.987. These data are representative of three independent experiments and the standard error for each individual rate constant has been estimated to be less than 8%.