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Two color FACS systems analysis using anti-BrdU and propidium iodide (PI) to determine cell proliferation. FITC Annexin V assay for identifying cells that are undergoing apoptosis. MTX-methotrexate., EA-early apoptosis, LA-late apoptosis, VC-viable cells, DC-dead cells, PI- propidium iodide. Values are mean ± SEM. * P<0.05 MTX-early vs non-treated Caco-2 cells, †P<0.05 MTX-late vs MTX- early.
