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. 2014 Nov 6;9(11):e112047. doi: 10.1371/journal.pone.0112047

Figure 2. bIgG binds to human FcγRII.

Figure 2

A) IgG was immobilised on 96 wells ELISA plates. Monocytes were incubated overnight in the absence or presence of 10 ng/ml IFN-γ. B) Different concentrations of IgG were coated and IFN-γ stimulated monocytes were added. C) Binding of PMNs to 10 µg/ml human (IVIg) and bovine IgG (bIg). PMNs were incubated for 10 minutes in the absence (–) or presence of 3 µg/ml Fabs recognizing FcγRIII (CD16) or FcγRII (CD32). As negative control, plates were coated with gelatin (none) instead of Ig. Binding of bIgG by monocytes and PMNs was observed for three different donors. For all panels binding of cells after five washes is shown and expressed as percentage of initial. Mean and S.E.M. is shown of triplicate measurements.