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. 2014 Nov 6;9(11):e112047. doi: 10.1371/journal.pone.0112047

Figure 3. Bovine IgG simultaneously binds to human RSV and human FcγRII.

Figure 3

Plates were coated with PEG-precipitated RSV, grown in HEp2 cells. A) Varying concentrations of human (IVIg) or bovine (bIgG) Ig were added, followed by incubation with calcein-labelled macrophages. A representative example of four donors tested is shown. No binding of cells was observed when Ig was inactivated prior to addition to the plate, or when Ig was added to gelatine coated plates (data not shown). B) 1000 µg/ml IVIg or bIgG was added to RSV-coated plates. PMNs were pre-incubated with medium, and/or anti FcγRII (CD32) or FcγRIII (CD16) Fabs. Independent experiments were performed with three donors. Graphs show mean and S.E.M. of triplicate measurements.