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. 2014 Nov 6;9(11):e112047. doi: 10.1371/journal.pone.0112047

Figure 6. Neutralisation of infection of HEp-2 cells by human RSV.

Figure 6

A) 5*10∧4 HEp-2 cells were seeded overnight in flat bottom 96-wells plates and infected the next day with 1*10∧5 PFU RSV-GFP which was pre-incubated with different concentrations of bovine (bIgG) or human (IVIg) IgG or monoclonal F-protein-specific palivizumab. GFP intensity was determined by flow cytometry as a measure for HEp2 cell infection by RSV-GFP. For the calculation of the inhibition percentage the MFI of uninfected cells was set to 100% and the MFI of infected cells without Ab incubation to 0%. Mean and S.E.M. of three independent experiments is shown. B) IC50 values for neutralisation of RSV-GFP by palivizumab, IVIg and bIgG are shown.