Salmonella
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YU39 (ST213)
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pA/C (150), p100 (100), pX1 (40), pColE1-like (5), p3 (3)
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Donor
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SO1 (ST19)
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pSTV::Km (94), p80 (80)
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Recipient
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LT2 (ST19)
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pSTV::Km (94)
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Recipient
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E. coli
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DH5α
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Recipient
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HB101
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Recipient
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HB101pSTV
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pSTV::Km
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Recipient
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DH5α
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pA/C
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Wild-type pA/C, donor
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DH5α
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pA/C, pSTV::Km
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Stability assays
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DH5α
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pX1
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Wild-type pX1
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Transconjugants
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SO1
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IA4
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pA/C
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Re-arranged pA/C
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IA5
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pA/C
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Re-arranged pA/C
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IA9
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pA/C
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Re-arranged pA/C
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IIA4
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pA/C + pX1
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pA/C and pX1 co-integrate
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HB101
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|
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IC2
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pX1::CMY
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pX1 with the transposed CMY region
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IIC1
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pX1::CMY
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pX1 with the transposed CMY region
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IIIC9
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pA/C + pX1
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pA/C and pX1 co-integrate
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IIIC10
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pX1::CMY
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pX1 with the transposed CMY region
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IVC8
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pA/C + pX1
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pA/C and pX1 co-integrate
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HB101pSTV
::Km
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|
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ID1
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pX1::CMY
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pX1 with the transposed CMY region
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IID2
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pX1::CMY
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pX1 with the transposed CMY region
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IIID8
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pA/C + pX1
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pA/C and pX1 co-integrate
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IVD2
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pA/C + pX1
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pA/C and pX1 co-integrate
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IVD8
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pX1::CMY
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pX1 with the transposed CMY region
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LT2
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|
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IIE2
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pX1::CMY
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pX1 with the transposed CMY region
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IIIE4
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pX1::CMY
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pX1 with the transposed CMY region
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IIIE9
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pA/C + pX1
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pA/C and pX1 co-integrate
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DH5α
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|
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221-1
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pA/C + pX1
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pA/C and pX1 co-integrate
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221-10
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pA/C + pX1
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pA/C and pX1 co-integrate
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225-1
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pA/C + pX1
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pA/C and pX1 co-integrate
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225-7
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pA/C + pX1
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pA/C and pX1 co-integrate
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pX1 mutants
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DH5α
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pX1ydgA::Tn5
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Tn5 transposon insertion
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DH5α
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pX1taxB::Km
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taxB site-directed mutant
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DH5α
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pA/C, pX1ydgA::Tn5
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Donor
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DH5α |
pA/C,pX1taxB::Km
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Donor |