Table 1.
Bacterial strains and plasmids used in this work
| Strain | Plasmids (kb) | Feature |
|---|---|---|
|
Salmonella
|
|
|
| YU39 (ST213) |
pA/C (150), p100 (100), pX1 (40), pColE1-like (5), p3 (3) |
Donor |
| SO1 (ST19) |
pSTV::Km (94), p80 (80) |
Recipient |
| LT2 (ST19) |
pSTV::Km (94) |
Recipient |
|
E. coli
|
|
|
| DH5α |
|
Recipient |
| HB101 |
|
Recipient |
| HB101pSTV |
pSTV::Km |
Recipient |
| DH5α |
pA/C |
Wild-type pA/C, donor |
| DH5α |
pA/C, pSTV::Km |
Stability assays |
| DH5α |
pX1 |
Wild-type pX1 |
|
Transconjugants |
|
|
|
SO1 |
|
|
| IA4 |
pA/C |
Re-arranged pA/C |
| IA5 |
pA/C |
Re-arranged pA/C |
| IA9 |
pA/C |
Re-arranged pA/C |
| IIA4 |
pA/C + pX1 |
pA/C and pX1 co-integrate |
|
HB101 |
|
|
| IC2 |
pX1::CMY |
pX1 with the transposed CMY region |
| IIC1 |
pX1::CMY |
pX1 with the transposed CMY region |
| IIIC9 |
pA/C + pX1 |
pA/C and pX1 co-integrate |
| IIIC10 |
pX1::CMY |
pX1 with the transposed CMY region |
| IVC8 |
pA/C + pX1 |
pA/C and pX1 co-integrate |
|
HB101pSTV
::Km
|
|
|
| ID1 |
pX1::CMY |
pX1 with the transposed CMY region |
| IID2 |
pX1::CMY |
pX1 with the transposed CMY region |
| IIID8 |
pA/C + pX1 |
pA/C and pX1 co-integrate |
| IVD2 |
pA/C + pX1 |
pA/C and pX1 co-integrate |
| IVD8 |
pX1::CMY |
pX1 with the transposed CMY region |
|
LT2 |
|
|
| IIE2 |
pX1::CMY |
pX1 with the transposed CMY region |
| IIIE4 |
pX1::CMY |
pX1 with the transposed CMY region |
| IIIE9 |
pA/C + pX1 |
pA/C and pX1 co-integrate |
|
DH5α |
|
|
| 221-1 |
pA/C + pX1 |
pA/C and pX1 co-integrate |
| 221-10 |
pA/C + pX1 |
pA/C and pX1 co-integrate |
| 225-1 |
pA/C + pX1 |
pA/C and pX1 co-integrate |
| 225-7 |
pA/C + pX1 |
pA/C and pX1 co-integrate |
|
pX1 mutants |
|
|
| DH5α |
pX1ydgA::Tn5 |
Tn5 transposon insertion |
| DH5α |
pX1taxB::Km |
taxB site-directed mutant |
| DH5α |
pA/C, pX1ydgA::Tn5 |
Donor |
| DH5α | pA/C,pX1taxB::Km | Donor |