Table 2.

First round conjugations for YU39 donor strain

Recipient strain	Transfer frequency a	No. transconjugants b	No. pA/C positive c	No. pX1 positive d	No. ColE1 e (% of total)
Typhimurium SO1 (pSTV::Km)	10-8 to 10-10	34	34	1	27 (79)
Typhimurium LT2 (pSTV::Km)	10-8 to 10-10	21	2	19	1 (0.4)
E. coli DH5α	10-7 to 10-9	10	10	10	5 (50)
E. coli HB101	10-7 to 10-8	28	9	21	4 (14)
E. coli HB101 (pSTV::Km)	10-8	28	8	24	4 (14)

^aThe frequency was calculated as number of transconjugants per donor; the range in the orders of magnitude obtained is shown.

^bNumber of transconjugants analyzed.

^cNumber of transconjugants positive for the repA/C PCR marker.

^dNumber of transconjugants positive for the oriX1 PCR marker.

^eNumber of transconjugants carrying pColE1-like.