Table 3.

Description of the pX1 :: CMY transconjugants obtained from the YU39 donor

Recipient	pX1 :: CMY colony	pX1 PCR typing						CMY region a	Insertion region b	Second round conjugation c
		oriX1	ydgA	taxB	taxC	ddp3	046-047			Original	DH5α
HB101	IC2	+	+	+	+	+	-	Large	046-047	10-1	1 to 10-2
	IIC1	+	-	+	+	+	+	Short	ND	10-1	10-1 to 10-2
	IIIC10	+	+	+	+	+	+	Short	stbE	10-1	10-1 to 10-2
HB101 (pSTV::Km)	ID1	+	+	+	+	+	-	Large	046-047	10-1	1 to 10-1
	IIID2	+	+	+	+	+	-	Large	046-047	10-2 to 10-4	10-4 to 10-7
	IVD8	+	+	+	+	+	+	Short	stbE	10-1 to 10-2	10-1
LT2	IIE2	+	+	+	+	+	+	Short	stbE	10-1 to 10-5	10-1
(pSTV::Km)	IIIE4	+	+	+	+	+	+	Large	ND	10-4 to 10-7	1 to 10-1

^aThe long CMY region includes from ISEcp1 to hypothetical protein 0093, and the short region includes from ISEcp1 to sugE (see text for details).

^bSection of pX1 where the CMY region was inserted (see text for details). ND, not determined.

^cTransconjugants were challenged for a second round of conjugation using as recipients the original recipient strain (original) or DH5α. The frequency was calculated as number of transconjugants per donor; the range in the orders of magnitude obtained is shown.