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. 2013 Nov 21;13:264. doi: 10.1186/1471-2180-13-264

Table 4.

Description of the pA/C and pA/C + pX1 transconjugants obtained from the YU39 donor

 
pX1 PCR typing
Second round conjugation a
Recipient pA/C colony oriX1 ydgA taxB taxC ddp3 046-047 Original DH5α
SO1
IA4
-
-
-
-
-
-
0b
0
 
IA5
-
-
-
-
-
-
0
0
 
IA9
-
-
-
-
-
-
0
0
 
IIIA4
+
+
+
+
+
+
10-8
1 to 10-2
DH5α
221-1
+
+
+
+
+
+
10-1 to 10-3
 
 
221-10
+
+
+
+
+
+
1 to 10-2
 
 
225-1
+
+
+
+
+
+
1 to 10-2
 
 
225-7
+
+
+
+
+
+
10-1 to 10-2
 
HB101
IIIC9
+
+
+
+
+
+
10-2 to 10-4
1 to 10-2
 
IVC8
+
+
+
+
+
+
10-2
10-1 to 10-2
HB101
IIID8
+
+
-
-
-
-
10-5
10-5 to 10-7
(pSTV::Km)
IVD2
+
+
+
+
+
+
1 to 10-2
0
LT2 (pSTV::Km) IIIE9 - - - - - - 0 10-4 to 10-7

aTransconjugants were challenged for a second round of conjugation using as recipients the original recipient strain (original) or DH5α. The frequency was calculated as number of transconjugants per donor; the range in the orders of magnitude obtained is shown.

bNo transconjugants were detected under the detection level (<10-10).