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. 2013 Nov 21;13:264. doi: 10.1186/1471-2180-13-264

Table 4.

Description of the pA/C and pA/C + pX1 transconjugants obtained from the YU39 donor

 
 pX1 PCR typing
 Second round conjugation a
Recipient pA/C colony oriX1 ydgA taxB taxC ddp3 046-047 Original DH5α
SO1
 IA4
 -
 -
 -
 -
 -
 -
 0b
 0
 
 IA5
 -
 -
 -
 -
 -
 -
 0
 0
 
 IA9
 -
 -
 -
 -
 -
 -
 0
 0
 
 IIIA4
 +
 +
 +
 +
 +
 +
 10-8
 1 to 10-2
DH5α
 221-1
 +
 +
 +
 +
 +
 +
 10-1 to 10-3
  
 
 221-10
 +
 +
 +
 +
 +
 +
 1 to 10-2
  
 
 225-1
 +
 +
 +
 +
 +
 +
 1 to 10-2
  
 
 225-7
 +
 +
 +
 +
 +
 +
 10-1 to 10-2
  
HB101
 IIIC9
 +
 +
 +
 +
 +
 +
 10-2 to 10-4
 1 to 10-2
 
 IVC8
 +
 +
 +
 +
 +
 +
 10-2
 10-1 to 10-2
HB101
 IIID8
 +
 +
 -
 -
 -
 -
 10-5
 10-5 to 10-7
(pSTV::Km)
 IVD2
 +
 +
 +
 +
 +
 +
 1 to 10-2
 0
LT2 (pSTV::Km) IIIE9 - - - - - - 0 10-4 to 10-7

aTransconjugants were challenged for a second round of conjugation using as recipients the original recipient strain (original) or DH5α. The frequency was calculated as number of transconjugants per donor; the range in the orders of magnitude obtained is shown.

bNo transconjugants were detected under the detection level (<10-10).