Table 5.
Conjugation experiments for pA/C and pX1 mutants using DH5α as recipient
| DH5α donor strain | Selection | Transfer frequency a | No. transconjugants c | No. pA/C positive d | No. pX1 positive e |
|---|---|---|---|---|---|
| pX1ydgA::Tn5 |
Km |
10-1 |
8 |
0 |
8 |
| pX1taxB::Km |
Km |
0b |
0 |
0 |
0 |
| pA/C, pX1ydgA::Tn5 |
Km |
1 to 10-2 |
4 |
0 |
4 |
| |
CRO |
10-5 to 10-7 |
6 |
6 |
6 |
| |
CRO-Km |
10-5 to 10-7 |
12 |
12 |
12 |
| pA/C, pX1taxB::Km |
Km |
0 |
0 |
0 |
0 |
| |
CRO |
0 |
0 |
0 |
0 |
| CRO-Km | 0 | 0 | 0 | 0 |
aThe frequency was calculated as number of transconjugants per donor; the range in the orders of magnitude obtained is shown.
bNo transconjugants were detected under the detection level (<10-10).
cNumber of transconjugants analyzed.
dNumber of transconjugants positive for the repA/C PCR marker.
eNumber of transconjugants positive for the oriX1 PCR marker.