Figure 1.

Relative steady-state ROS levels. ROS measured in relative fluorescence units of PQ-treated nematodes fed live E. coli and raised at either 20°C (A) or 25°C (B). Filled symbols show ROS levels measured at earlier, developmentally similar (larval) time points (34 h at 20°C and 24 h at 25°C); open symbols show later, mostly developmentally similar (reproductively mature) time points (68 h at 20°C and 48 h at 25°C) for each assay. Note that the data point for older individuals in the High PQ treatment at 25°C (grey filled symbol) should be interpreted with caution due to the extremely delayed development of this group, and that the change in scale between the y-axes of (A) and (B) is not meaningful (see Results). Bars show one SEM. ANOVA revealed effects of PQ level (F_{3, 316} = 3.320, P = 0.0202), age (F₁ = 440.1, P < 0.0001), and PQ x age (F₃ = 21.51, P < 0.0001) on ROS levels measured at 20°C. The same was true for the 25°C assay with significant effects of PQ level (F_{3, 319} = 63.39, P < 0.0001), age (F₁ = 36.71, P < 0.0001) and PQ x age (F₃ = 67.33, p < 0.0001) on ROS level.