Table 1. Primer sequences used for real-time polymerase chain reaction analysis.

Gene	Primer sequence	Product size(bp)	Annealing Temperature (°C)	Cycles
FSHr	(f) 5′-TGCTACACCCACATCTACCT-3′	307	60	32
	(r) 5′-GCACCTCATAACAGCCAAAC-3′
LHr	(f) 5′-ATTCAGACGCTCATCGC-3′	370	56.6	38
	(r) 5′-GCCAAATCAACACCCTAA-3′
GnRHr	(f) 5′-ATCCCTTTGACTTTCACATCC-3′	184	61	35
	(r) 5′-CACCTCCTTGCCCATCTC-3′
FSHβ	(f) 5′-TCCCTCCATCCATACTGT-3′	463	56	32
	(r) 5′-GCTTGGTTACTACCTCCTG-3′
LHβ	(f) 5′-CGGCCTGTCAACGCAACT-3′	110	60	40
	(r) 5′-GGCAGTACTCGGACCATGCT-3′
GnRH1	(f) 5′-ATCCCTTTGACTTTCACATCC-3′	184	61	32
	(r) 5′-CACCTCCTTGCCCATCTC-3′
Kiss-1	(f) 5′-CCACCTACAACTGGAACTCCTTCG-3′	110	60	34
	(r) 5′-CCCTGCCTTGGCCTCTACAATC-3′
Gpr54	(f) 5′-GGCTCCGTCCAACGCTTCAG-3′	174	64	32
	(r) 5′-TGTGCTTGTGGCGGCAGATA-3′
18s rRNA	(f) 5′-AGGGGAGAGCGGGTAAGAGA-3′	241	60	27
	(r) 5′-CACCTCCTTGCCCATCTC-3′

Abbreviation: FSH: folliclestimulating hormone, LH: luteinising hormone, GnRH: gonadotropin releasing hormone, RNA: ribonucleic acid.