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. 2013 Nov 25;13:188. doi: 10.1186/1471-2229-13-188

Figure 3.

Figure 3

Subcellular localization analysis of BcWRKYs. The subcellular localization analysis of the putative BcWRKY proteins used NLStradamus, NucPred, and WOLF PSORT with the default settings to detect nuclear localization scores. (A) two yellow fluorescent protein (YFP) marker expressing BcWRKY fusion genes, BcWRKY25-YFP and BcWRKY40-YFP, were constructed; (B) BcWRKY25-YFP and BcWRKY40-YFP were introduced into onion epidermal cells by particle bombardment with the YFP signal as an indicating marker to test the subcellular localization of BcWRKY proteins; (C) the upper panel, the corresponding bright field, fluorescence, merged fluorescence image of YFP control; the middle panel, the corresponding bright field, fluorescence, merged fluorescence image of BcW25-YFP; the lower panel, the corresponding bright field, fluorescence, merged fluorescence image of BcW40-YFP. Scale bars: 20 μm.