Table 1.
Identification of stress-inducible WRKY genes in Pak-choi
| Gene name | Accession number | CDS (bp) | Size (aa) | Mass (KDa) | pI | Group | Atortholog | NLS locaton | NucPred score | Nuclear localization |
|---|---|---|---|---|---|---|---|---|---|---|
|
BcWRKY1 |
KF430025 |
858 |
285 |
31.75 |
7.64 |
IIa |
AtWRKY18 |
95-98 |
0.85 |
Nucl: 13.0 |
|
BcWRKY2 |
KF430026 |
2067 |
860 |
93.24 |
7.53 |
I |
AtWRKY2 |
152-170 |
0.84 |
Nucl: 14.0 |
|
BcWRKY3 |
KF430027 |
1428 |
475 |
51.92 |
7.94 |
I |
AtWRKY3 |
|
0.86 |
Nucl: 13.0 |
|
BcWRKY4 |
KF430028 |
1488 |
495 |
53.79 |
8.01 |
I |
AtWRKY4 |
339-351 |
0.89 |
Nucl: 14.0 |
|
BcWRKY6 |
KF430029 |
1662 |
550 |
60.34 |
5.94 |
IIb |
AtWRKY6 |
267-268 |
0.63 |
Nucl: 13.0 |
|
BcWRKY7 |
KF430030 |
1047 |
348 |
38.02 |
10.42 |
IId |
AtWRKY7 |
253-273 |
0.82 |
Nucl: 12.0 |
|
BcWRKY8 |
KF430031 |
966 |
379 |
42.58 |
7.14 |
IIc |
AtWRKY8 |
189-226 |
0.72 |
Nucl: 13.0 |
|
BcWRKY9 |
KF430032 |
987 |
328 |
37.38 |
5.55 |
IIb |
AtWRKY9 |
113-125 |
0.75 |
Nucl: 12.5 |
|
BcWRKY11 |
KF430033 |
999 |
332 |
36.37 |
10.18 |
IId |
AtWRKY11 |
226-249 |
0.92 |
Nucl: 14.0 |
|
BcWRKY12 |
KF430034 |
657 |
218 |
24.62 |
8.73 |
IIc |
AtWRKY12 |
112-133 |
0.49 |
Nucl: 4.0 |
|
BcWRKY13 |
KF430035 |
882 |
293 |
32.97 |
9.36 |
IIc |
AtWRKY13 |
169-201 |
0.74 |
Nucl: 13.0 |
|
BcWRKY15 |
KF430036 |
960 |
319 |
34.71 |
10.40 |
IId |
AtWRKY15 |
217-237 |
0.86 |
Nucl: 11.0 |
|
BcWRKY18 |
KF430037 |
969 |
317 |
35.69 |
8.32 |
IIa |
AtWRKY18 |
25-32 |
0.94 |
Nucl: 13.0 |
|
BcWRKY20 |
KF430038 |
1608 |
535 |
58.64 |
6.96 |
I |
AtWRKY20 |
|
0.89 |
Nucl: 14.0 |
|
BcWRKY21 |
KF430039 |
1020 |
339 |
38.04 |
10.28 |
IId |
AtWRKY21 |
247-267 |
0.95 |
Nucl: 14.0 |
|
BcWRKY22 |
KF430040 |
897 |
298 |
32.34 |
7.16 |
IIe |
AtWRKY22 |
101-121 |
0.66 |
Nucl: 13.0 |
|
BcWRKY23 |
KF430041 |
972 |
323 |
36.10 |
6.91 |
IIc |
AtWRKY23 |
133-152 |
0.74 |
Nucl: 13.0 |
|
BcWRKY24 |
KF430042 |
531 |
176 |
20.44 |
8.62 |
IIc |
AtWRKY24 |
67-87 |
0.5 |
Nucl: 6.0 |
|
BcWRKY25 |
KF430043 |
1122 |
373 |
42.06 |
6.68 |
I |
AtWRKY25 |
206-223 |
0.58 |
Nucl: 13.0 |
|
BcWRKY26 |
KF430044 |
894 |
319 |
35.94 |
9.62 |
I |
AtWRKY26 |
217-230 |
0.64 |
Nucl: 12.5 |
|
BcWRKY28 |
KF430045 |
939 |
312 |
34.97 |
6.72 |
IIc |
AtWRKY28 |
116-156 |
0.69 |
Nucl: 13.0 |
|
BcWRKY29 |
KF430046 |
927 |
308 |
34.17 |
8.43 |
IIe |
AtWRKY29 |
108-131 |
0.55 |
Nucl: 13.0 |
|
BcWRKY30 |
KF430047 |
942 |
313 |
35.57 |
6.80 |
III |
AtWRKY30 |
97-104 |
0.52 |
Nucl: 11.5 |
|
BcWRKY31 |
KF430048 |
1560 |
519 |
57.15 |
6.95 |
IIb |
AtWRKY42 |
212-217 |
0.69 |
Nucl: 14.0 |
|
BcWRKY32 |
KF430049 |
1899 |
459 |
49.58 |
6.97 |
I |
AtWRKY26 |
300-311 |
0.61 |
Nucl: 11.0 |
|
BcWRKY33 |
KF430050 |
1557 |
518 |
56.63 |
7.92 |
I |
AtWRKY33 |
|
0.77 |
Nucl: 13.0 |
|
BcWRKY34 |
KF430051 |
1650 |
549 |
60.18 |
6.47 |
I |
AtWRKY34 |
19-28 |
0.78 |
Nucl: 14.0 |
|
BcWRKY36 |
KF430052 |
1152 |
383 |
42.99 |
7.69 |
IIb |
AtWRKY36 |
96-129 |
0.74 |
Nucl: 12.0 |
|
BcWRKY39 |
KF430053 |
1029 |
342 |
38.00 |
10.02 |
IId |
AtWRKY74 |
247-271 |
0.9 |
Nucl: 11.0, |
|
BcWRKY40 |
KF430054 |
873 |
290 |
32.43 |
7.82 |
IIa |
AtWRKY40 |
75-89 |
0.72 |
Nucl: 10.0 |
|
BcWRKY42 |
KF430055 |
1551 |
519 |
57.15 |
6.95 |
IIb |
AtWRKY42 |
212-217 |
0.69 |
Nucl: 14.0 |
|
BcWRKY44 |
KF430056 |
1230 |
409 |
44.89 |
9.19 |
I |
AtWRKY44 |
360-364 |
0.67 |
Nucl: 8.0 |
|
BcWRKY46 |
KF430057 |
858 |
283 |
32.20 |
5.82 |
III |
AtWRKY46 |
60-90 |
0.73 |
Nucl: 10.0 |
|
BcWRKY47 |
KF430058 |
1512 |
490 |
53.94 |
6.79 |
IIb |
AtWRKY47 |
|
0.77 |
Nucl: 13.0 |
|
BcWRKY48 |
KF430059 |
1200 |
399 |
44.46 |
6.77 |
IIc |
AtWRKY48 |
185-212 |
0.69 |
Nucl: 13.0 |
|
BcWRKY51 |
KF430060 |
597 |
198 |
22.29 |
7.54 |
IIc |
AtWRKY51 |
112-129 |
0.49 |
Nucl: 12.0 |
|
BcWRKY53 |
KF430061 |
972 |
323 |
36.19 |
6.38 |
III |
AtWRKY53 |
127-149 |
0.81 |
Nucl: 14.0 |
|
BcWRKY54 |
KF430062 |
1020 |
297 |
33.11 |
4.76 |
III |
AtWRKY54 |
107-131 |
0.8 |
Nucl: 12.0 |
|
BcWRKY56 |
KF430063 |
564 |
186 |
21.03 |
7.23 |
IIc |
AtWRKY56 |
79-89 |
0.37 |
Nucl: 9.0 |
|
BcWRKY57 |
KF430064 |
885 |
294 |
32.56 |
6.89 |
IIc |
AtWRKY57 |
116-141 |
0.67 |
Nucl: 13.0 |
|
BcWRKY58 |
KF430065 |
3195 |
1082 |
119.84 |
9.63 |
I |
AtWRKY58 |
865-913 |
0.96 |
Nucl: 14.0 |
|
BcWRKY59 |
KF430066 |
546 |
196 |
22.64 |
6.95 |
IIc |
AtWRKY59 |
75-97 |
0.84 |
Nucl: 5.0 |
|
BcWRKY60 |
KF430067 |
927 |
308 |
34.71 |
8.22 |
IIa |
AtWRKY18 |
103-109 |
0.91 |
Nucl: 13.0 |
|
BcWRKY61 |
KF430068 |
1662 |
398 |
44.24 |
6.44 |
IIb |
AtWRKY61 |
|
0.66 |
Nucl: 12.0 |
|
BcWRKY62 |
KF430069 |
837 |
233 |
26.92 |
6.27 |
III |
AtWRKY62 |
57-73 |
0.61 |
Nucl: 13.0 |
|
BcWRKY64 |
KF430070 |
777 |
258 |
29.36 |
4.82 |
III |
AtWRKY67 |
108-117 |
0.67 |
Nucl: 11.0 |
|
BcWRKY65 |
KF430071 |
786 |
261 |
29.25 |
5.27 |
IIe |
AtWRKY65 |
133-150 |
0.55 |
Nucl: 13.0 |
|
BcWRKY66 |
KF430072 |
738 |
452 |
52.12 |
6.96 |
III |
AtWRKY64 |
121-138 |
0.56 |
Nucl: 8.0 |
|
BcWRKY67 |
KF430073 |
831 |
276 |
31.06 |
6.79 |
III |
AtWRKY70 |
92-118 |
0.81 |
Nucl: 11.0 |
|
BcWRKY68 |
KF430074 |
960 |
319 |
36.04 |
6.61 |
IIc |
AtWRKY23 |
116-145 |
0.82 |
Nucl: 13.0 |
|
BcWRKY69 |
KF430075 |
849 |
282 |
31.27 |
4.69 |
IIe |
AtWRKY69 |
32-41 |
0.46 |
Nucl: 13.0 |
|
BcWRKY70 |
KF430076 |
852 |
283 |
32.01 |
6.23 |
III |
AtWRKY70 |
92-117 |
0.77 |
Nucl: 12.0 |
|
BcWRKY71 |
KF430077 |
1047 |
277 |
31.53 |
8.45 |
IIc |
AtWRKY71 |
99-125 |
0.46 |
Nucl: 11.0 |
|
BcWRKY72 |
KF430078 |
2529 |
551 |
59.68 |
6.36 |
IIb |
AtWRKY72 |
|
0.58 |
Nucl: 14.0 |
|
BcWRKY74 |
KF430079 |
969 |
322 |
35.29 |
10.45 |
IId |
AtWRKY11 |
211-244 |
0.96 |
Nucl: 13.0 |
| BcWRKY75 | KF430080 | 1062 | 353 | 39.33 | 6.85 | IIc | AtWRKY75 | 143-167 | 0.73 | Nucl: 13.0 |
Abbreviations: bp, base pair; aa, amino acids; CDS, coding sequence; pI, Isoelectric point; WD, WRKY domain; NLS, Nuclear location signal. NLS location indicated the distribution of the NLSs on the BcWRKY proteins.