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. 2014 Jul 25;11:59. doi: 10.1186/1742-4690-11-59

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© Young et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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RE expression in the gut is dependent on genotype and husbandry conditions. (A) Heatmap of RE-reporting probes significantly regulated between GF and SPF housing conditions (p < 0.01 by ANOVA comparing husbandry conditions, eliminating genotype and tissue), using data from E-GEOD-17438, a Mouse Gene 1.0ST array. Each column is a single tissue from a single mouse. Intestinal tissues are separated with vertical lines in anatomical order: duodenum, jejunum, ileum and colon. Probes reporting MMTV, MLV, and Emv2 expression are highlighted. (B) qRT-PCR analysis of eMLV expression between Myd88-deficient and -sufficient B6 mice housed in SPF or GF facilities. Values exceeding 10³ are considered high and are colored red.