Table 2.

Sequences of primers and PCR conditions

	Sequences of primers	PCR conditions
Reverse transcription	5′-TGR TGC ACG GTC TAC GAG ACC TC-3′	Temp. 72°C/30 min
I PCR	5′-RAYCACTCCCCTGTGAGGAAC-3′	1 min at 94°C;
		50 cycles of 94°C for 15 s, 58°C for 30 s, and 72°C for 30 s; 72°C for 7 min
II PCR	5′-ACTGTCTTCACGCAGAAAGCGTC-3′	1 min at 94°C;
	5′-CAAGCACCCTATCAGGCAGTACC-3′	30 cycles of 94°C for 15 s, 58°C for 1 min, and 72°C for 1 min; at 72°C for 7 min