Table 1. Primer Sequences for Real-Time PCR.
Species | Genes | Primer sequences (5′ – 3′) | |
Sense | Antisense | ||
human | NRF-1 | CCAAGTGAATTATTCTGCCG | TGACTGCGCTGTCTGATATCC |
SCO1 | GGCACAGCCAGTGCATTCCTGCCTG | GCATCACACTCGTGATCAATATCCTC | |
SCO2 | GCAGCCTGTCTTCATCACTGTGGACC | CCGCACACTGTCTGAGATCTGCTC | |
TFAM | AGCTAAGGGTGATTCACCGC | GCAGAAGTCCATGAGCTGAA | |
COX IV | ACGAGCTCATGAAAGTGTTGTG | AATGCGATACAACTCGACTTTCTC | |
HPRT1 | GACACTGGCAAAACAATGCAG | AGTCTATAGGCTCATAGTGC | |
MFN1 | AGTAACAGGATTGGCGTCCG | CGTTTCCTCCTATCATGGTCACC | |
MFN2 | ATGCATCCCCACTTAAGCAC | CCAGAGGGCAGAACTTTGTC | |
OPA1 | GGCTCTGCAGGCTCGTCTCAAGG | TTCCGCCAGTTGAACGCGTTTACC | |
DRP1 | CACAGGAGGAGGTGGACAGC | CGCCTCCTTCAGTGCGTGGT | |
FIS1 | ATGGAGGCCGTGCTGAAC | TCAGGATTTGGACTTGGA | |
mouse | PGC-1α | ACGGTTTACATGAACACAGCTGC | CTTGTTCGTTCTGTTCAGGTGC |
NRF-1 | GAACGCCACCGATTTCACTGTC | CCCTACCACCCACGAATCTGG | |
TFAM | CTGATGGGTATGGAGAAGGAGG | CCAACTTCAGCCATCTGCTCTTC | |
p53R2 | CCAGGTTACCATGGTTGTGG | CCAGTGCACTCAGTAGCTGTG | |
SCO1 | CTAGCTTAGCACAATAGCAAGGGCAGGCTAC | CCCAGGAATGCAGTTATGACATGACAGCAAAGGCAG | |
SCO2 | CAGCCTGTCTTCATCACTGTGGA | GACACTGTGGAAGGCAGCTATGTGCC | |
TIF | CTGAGGATGTGCTGTCTGGGAA | CCTTTGCCTCCACTTCGGTC |