Table 2. Oligonucleotides used in this study.
| Oligonucleotide Primers | Sequence (5′-3′)a |
| Recombinant expression of GST-Har and variants | |
| HarNterm | CGGAATTCATCGAAGGTCGTATGATAGTCACATCA |
| HarCterm | CGCTCGAGCCATATCGGATCAATGTTATATGTCT |
| Har150_Nterm | CCGCGTGGATCCATGATAGTCACATCACTG |
| Har150_Cterm | CGTGGATCCCGGGTTACTGCTTCTTCCTGCATTT |
| HarC97A SDM | GCTTCATTTGCAGAGCAGGCACCGCTGCTTTTCTGTACC |
| DNA target for EMSA | |
| PGN_0001_240bp_For | GGTGTTGATAACTCGGTCGCGCCTT |
| PGN_0001Rev | CTAAAAAAATATCGTTTTGAGAGCAGT |
| Construction of har mutant | |
| PGN_1502-Fwd | ATGTCGCCTTCCGAGGCTAT |
| ErmF-PGN_1502-Rev | GCAATAGCGGAAGCTATCGGTTATCTTTTCGATCCATTCTTGC |
| PGN_1502-ErmF-Fwd | AGAATGGATCGAAAAGATAACCGATAGCTTCCGCTATTGC |
| Term-ErmF-Rev | GTCTTTCGACTGAGCCTTTCGTTTTAGCATCTAATTTAACTTCAATTCC |
| Term-Prom-region-Fwd | GCTCAGTCGAAAGACTGGGCCTTTCGTTTTACGGAGTGAAAAAGGAGCCG |
| PGN_1504-Prom-region-Rev | CAATGTTATATGTCTGTGTTATCTCTCTTTTACATCATATTTTCC |
| Prom-region-PGN_1504-Fwd | AATATGATGTAAAAGAGAGATAACACAGACATATAACATTGATCC |
| PGN_1504-Rev | GCAGATATTTTGTAGCCTCCATC |
| Construction of har complement | |
| PGN_1502-Fwd | ATGTCGCCTTCCGAGGCTAT |
| CepA-Har-Rev | ACTTTCCTTAACTCTTTTGACGTCTTATTTTTTCTTCTTGGGAGCGGCT |
| Har-CepA-Fwd | AGCCGCTCCCAAGAAGAAAAAATAAGACGTCAAAAGAGTTAAGGAAAGT |
| ErmF-CepA-Rev | TGTGTAGGTTCTAATTGAAGGACAGACGTCTCAAGTCACCGATAG |
| CepA-ErmF-Fwd | CTATCGGTGACTTGAGACGTCTGTCCTTCAATTAGAACCTACACA |
| ErmF-Rev | GATACTGCACTATCAACACACTC |
| qRT-PCR | |
| PGN_0287 RT Fwd | CCAGCAGCACTTTCCATACAAA |
| PGN_0287 RT Rev | CCACTGATTACGGCCTCATTT |
| PGN_0448 RT Fwd | AGTAAAGGGGTAGGGCAACG |
| PGN_0448 RT Rev | ATCGGATTCGTGTTCCAAAGC |
| PGN_1296 RT Fwd | CCTGCAAGAGCGTGAAGTTG |
| PGN_1296 RT Rev | GGATCGGAAAGCCGTATAAGC |
| PGN_1578 RT Fwd | TGTTGTGGAAAGGAGTGTGG |
| PGN_1578 RT Rev | AGAAGGAATGAAGTCGGTTGTT |
| PGN_2083 RT Fwd | GCATTCTTTTCTGGCGTAGCA |
| PGN_2083 RT Rev | TTTGCGAAACGGCACTCCCT |
a
Underlined sequence of SOE primers indicates the part of the primer that is complementary to the target sequence, with the remainder of the primer providing complementarity with a second PCR product for splicing.