Table 4. Zinc ions released from Zn(II)Har and Zn(II)Har150 by the cysteine oxidising reagent DTNB.
| Without DTNB | With DTNB | |||
| Protein sample | Zn(II)Har | Zn(II)Har150 | Zn(II)Har | Zn(II)Har150 |
| Protein conc. (µM) a | 13.21 | 7.31 | 13.21 | 7.31 |
| Zn in filtrate (µM) | nd c | nd | 11.39 | 5.88 |
| Zn/P b | - | - | 0.86 | 0.81 |
Zinc content in the filtrates of Zn(II)Har and Zn(II)Har150 after 15 min incubation of the proteins with and without DTNB (160 µM) in 100 mM sodium phosphate pH 8.0 as determined by ICP-MS.
a
before centrifugation through the 3 kDa MWCO filter.
b
the ratio of zinc content in the filtrate to the protein concentration before centrifugation.
c
not detected.