Figure 2. Binding of human recombinant tissue factor (TF) by three commercial anti-TF antibodies (2K1, 4G4 and GMA) evaluated by western blot (upper panel) and enzyme immunoassay (lower panel) methods.

Only 2K1 efficiently recognizes TF with both methods. The last lane of western blotting refers to the size markers (SM). In enzyme immunoassay experiments, data represent the mean of three different measurements.