Table 2. Expression of target (tissue factor) and housekeeping (beta-actin) genes in purified eosinophils obtained from 9 patients with hypereosinophilia and 9 normal controls.

N	Condition	Tissue factor Ct	Beta-actin Ct	2ΔCt
	Patients with hypereosinophilia
1	Bullous pemphigoid	36.50	31.27	0.43
2	Asthma with eosinophilia	35.20	36.31	2.16
3	Bullous pemphigoid	33.72	31.56	0.45
4	Churg-Strauss syndrome	35.10	31.04	0.51
5	Asthma with eosinophilia	34.91	28.98	0.52
6	Strongyloidiasis	35.67	33.12	0.37
7	Ascariasis	35.54	30.10	0.49
8	Idiopathic hypereosinophilic syndrome	19.45	27.79	20.25
9	Idiopathic hypereosinophilic syndrome	33.68	32.25	0.56
	Median	35.10 *	31.27	0.51**
	Range	19.45–36.50	27.79–36.31
	Normal controls
1	Healthy	37.18	31.66	0.02
2	Healthy	36.04	33.65	0.19
3	Healthy	35.33	33.23	0.23
4	Healthy	37.87	30.47	0.01
5	Healthy	37.25	33.89	0.10
6	Healthy	36.62	31.76	0.03
7	Healthy	37.17	29.57	0.01
8	Healthy	37.23	31.95	0.03
9	Healthy	36.12	30.28	0.02
	Median	37.17	31.76	0.03
	Range	35.33–37.87	29.57–33.89	0.01–0.23

Gene expression was analysed by real-time polymerase chain reaction and reported as cycle threshold (Ct) and as corrected Ct (2^ΔCt = 2^{Ct tissue factor - Ct beta actin}).

* Median value of tissue factor cycle threshold (Ct) was significantly lower in patients with hypereosinophilia than in healthy subjects (p = 0.002). **Median value of tissue factor Ct corrected for beta actin Ct (using the equation 2^ΔCt = 2^{Ct tissue factor - Ct beta actin}) was significantly higher in patients with hypereosinophilia than in healthy subjects (p = 0.0001). Both analyses indicate an increased mRNA espression of tissue factor in patients with hypereosinophilia.