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. 2014 Nov 6;9(11):e112076. doi: 10.1371/journal.pone.0112076

Figure 3. Effects of suspension on fly walking.

Figure 3

A. Trajectory of leg motions (blue lines) in unperturbed flies (Con). Black dots are touchdowns. Immediately after suspension (“0 min”), the legs retrogress (red line). After 2 hours (120 min) retrogression is reduced. B. Backtracking at different times after release from suspension. Note that the initial retrogression gradually subsides. Dotted line represents retrogression in Con. n = 12. * p<0.05, One way repeated measures ANOVA with post-hoc Dunnett multiple comparison test. C. The effects of suspension and amputation are additive. The effect of suspension (Susp), amputation(Amp) and their combination (Susp+Amp) was defined as the difference between the initial (0 min) and the final (120 min) values. n = 12–17. p<0.0001, Kruskal-Wallis test. * p<0.05 *** p<0.001, Mann-Whitney test with bonferroni correction of p values. Error bars are mean ±SEM.