Skip to main content
. 2014 Nov 6;9(11):e112336. doi: 10.1371/journal.pone.0112336

Figure 5. DACT2 inhibit Cell migration and invasion by inhibiting Wnt pathway in TPC-1 cells.

Figure 5

A: Results of TCF/LEF luciferase reporter assay. β-catenin expression vector was co-transfected with TCF/LEF Topflash reporter or its mutant, Fopflash in TPC-1 cells. Luciferase activity was normalized to Renilla luciferase activity. Relative luciferase activity (the ratio of firefly luciferase to renilla luciferase) was suppressed by DACT2. The experiment was repeated for three times (*p<0.05). Increased luciferase activity was induced by co-transfecting DACT2 and Dvl2. The experiment was repeated for three times (*p<0.05). B: Cell migration and invasion was increased by β-catenin in DACT2 stably expressed TPC-1 cells under the transwell study. The experiment was repeated for three times (p<0.05). C: The expression of β-catenin, c-myc, cyclinD1 and MMP-9 were decreased and the level of phosphorylated β-catenin(p-β-catenin) was increased after the restoration of DACT2 expression in TPC-1 cells. The expression of β-catenin, c-myc, cyclinD1 and MMP-9 were increased and the level of p-β-catenin was reduced after knock down DACT2 in W3 and SW579 cells.